McAllister Muenster, Kesik Brodacka

ABSTRACT

Background: A fast, reliable, and sensitive RP-HPLC method was developed for the simultaneous quantification of Lamivudine (3TC), Tenofovir (TFV), and Efavirenz (EFV) in pure form and their combined pharmaceutical formulations. Methods: The analysis was performed on an Inertsil ODS-3V column (250 × 4.6 mm, 5 ?m) using an isocratic mobile phase composed of Acetonitrile and 1% Isopropyl Alcohol (IPA) in the ratio 85:15, flowing at 1 mL/min. Detection was carried out at 256 nm with a PDA detector