Halavath Ramesh* and M. F. Valan

ABSTRACT

The parts of the Nicotiana tabacum plant have been known to possess a wide range of biological activities. In present study, Nicotina Tobaccum leaves were extracted by using N-hexane, chloroform and ethyl acetate (EA) and these extracts were studied for a phytochemicals investigation, antibacterial activity and antioxidant activity. A new route of preparation of nicotine has been tried and found to be successful. The product was purified by thin layer chromatography and characterized by IR spectrum. HPTLC method identified total phytochemical constituents. The various functional groups, detected from FT-IR analysis of N-hexane, chloroform and EA extracts, are responsible for the medicinal properties of plant. The compounds identified by GC-MS in solvent extract of Nicotina Tobaccum are medicinally valuable and possess a wide variety of pharmacological applications. HRMS technique quantified the N-Hexane solvent extract. Further isolation and testing of phytoconstituents for their individual biological activity will undoubtedly bring promising results in the discovery and development of novel drugs. Keywords: Nicotiana tabacum, Biological activities, N-hexane, Chloroform, Ethyl Acetate, Nicotine, Phytochemical Constituents