A. A. Makhani* and L. D. Devhare

ABSTRACT

UV spectrophotometric techniques have been developed and validated for the simultaneous determination of drotaverine and nimesulide in synthetic mixture and its combined dosage form. The method was based on UVspectrophotometric determination of two drugs using Vierordt’s simultaneous equation method. This employs formation and solving of mathematical simultaneous equation using 229 nm and 297 nm as the ?max of drotaverine and nimesulide respectively in methanol. The % assay for commercial formulation was found to be in the range 100.07 ± 0.22 % for drotaverine and 99.62±0.19 % for nimesulide by the proposed methods. Recovery was found in the range 99.90±0.11 % for drotaverine and 100.01±0.25% for nimesulide. The methods were validated in terms of linearity, precision, accuracy and ruggedness according to ICH guideline. The methods were successfully applied to pharmaceutical formulation with no interference from excipients. This method can be adapted to routine quality control analysis of drugs in combined dosage form. Keyword: Drotaverine, Nimesulide, UV- Spectrophotometric and simultaneous estimation.